Good work, great potential
I thought there were some very good parts to this episode, including the opening
"hotdog" scene, the epic punch scenes, the shooting of the two girls who finish each other's sentences, and the unveiling of swirly glasses' final form.
The animation and art was also great, as was the sound quality and voice acting. Perhaps you could play with camera angles a bit more when characters talk (since this is something anime series sometimes do as well), but that's more of a stylistic choice.
In terms of humor, while I thought the breaking of the fourth wall after one of the characters died was an interesting choice, I'm not sure if it worked in the cartoon. For me, it slowed down the rapid-fire pacing, and was not as funny as the rest of the jokes in the piece.
For future episodes, you also may want to consider adding background music during the episode, and perhaps more specific anime references. I think music could really add to your scenes and flow, and would give you another facet of anime to parody.
And while you did throw in a few specific anime references that I caught on to -- such as the punch scenes and the opening hotdog scene -- it seemed most of the cartoon dealt with more general aspects of anime, such as the fact girls have large breasts, the nerdy character suddenly becoming powerful, the reusing of animation and poses, etc.
Even though that's all well and good, I thought the more specific references were your funniest jokes, rather than the general references, which are less original and a bit more stale. Plus, Considering the audience, you could probably add more specific jokes while still allowing your cartoon to be accessible and original.
In the end, however, very good work, and I look forward to future episodes.